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M9490419.TXT
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1994-09-19
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Document 0419
DOCN M9490419
TI Detection and characterization of a functional complex of human
immunodeficiency virus type 1 integrase and its DNA substrate by UV
cross-linking.
DT 9411
AU Yoshinaga T; Kimura-Ohtani Y; Fujiwara T; Shionogi Institute for Medical
Science, Osaka, Japan.
SO J Virol. 1994 Sep;68(9):5690-7. Unique Identifier : AIDSLINE
MED/94335083
AB To analyze the association of the human immunodeficiency virus type 1
integrase (IN) protein with DNA substrates, we applied a shortwave UV
cross-linking method to the in vitro integration system. Three
photoadduct bands were detected on sodium dodecyl sulfate-polyacrylamide
gels. The appearances of these photoadducts were examined with various
substrates and under several incubation conditions. Our data suggest
that one of these photoadducts is derived from the sequence- and
strand-specific bound state of the early phase of the integration
reaction before the 3' processing reaction. We also show that most of
the photoadduct complexes are competent for integration even after UV
irradiation.
DE Base Sequence DNA Nucleotidyltransferases/*METABOLISM DNA-Binding
Proteins/METABOLISM DNA, Viral/*METABOLISM Ethylmaleimide/CHEMISTRY
HIV-1/*ENZYMOLOGY Molecular Sequence Data
Oligodeoxyribonucleotides/CHEMISTRY Photochemistry Structure-Activity
Relationship Time Factors Ultraviolet Rays *Virus Integration
JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).